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fixation buffer (Thermo Fisher)

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Thermo Fisher fixation buffer
Fixation Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fixation+buffer/HEPES/pm42001993-277-4-62
Average 99 stars, based on 1 article reviews

fixation buffer - by Bioz Stars, 2026-09

99/100 stars

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Incubation:

Article Title: STAT3 inhibition in combination with CD47 blockade inhibits osteosarcoma lung metastasis.
Article Snippet: .. After incubation, cells were washed in FACS buffer and fixed using fixation buffer (Thermofisher). .. For intracellular/intranuclear staining, fixed cells were washed with permeabilization buffer followed by staining with antibody in the buffer for 30 mins at 4 C. Cells were acquired using BD Fortessa and analyzed using FlowJo v. 10 software. frontiersin.org

Article Title: A fluorescent sensor for real-time monitoring of DPP8/9 reveals crucial roles in immunity and cancer
Article Snippet: .. After the treatment/induction period, the media were removed, and cells were washed with 1 ml PBS and incubated with 1 ml fixation buffer (4% PFA in PBS; Thermo Fisher Scientific) for 15 min at RT. .. Then, cells were washed 3x with 1 ml PBS and incubated with 0.5 ml of 4′,6-diamidino-2-phenylindole (DAPI) solution (1 μg/ml; Roth) for 15 min at RT.

Article Title: A fluorescent sensor for real-time monitoring of DPP8/9 reveals crucial roles in immunity and cancer.
Article Snippet: .. After the treatment/induction period, the media were removed, and cells were washed with 1 ml PBS and incubated with 1 ml fixation buffer (4% PFA in PBS; Thermo Fisher Scientific) for 15 min at RT. .. Then, cells were washed 3x with 1 ml PBS and incubated with 0.5 ml of 49,6-diamidino-2phenylindole (DAPI) solution (1 μg/ml; Roth) for 15 min at RT.

Article Title: Influence of Insert Brand and Culture Method on Ciliary Activity and Epithelial Cell Types in Human Nasal Air–Liquid Interface Cell Cultures
Article Snippet: .. After incubation for 20 min on ice, cells were washed once with FACS buffer and fixed in 100 μL Fixation buffer (ThermoFisher, #00-8222-49) for 15 min on ice. ..

FACS:

Staining:

Article Title: Phospho-mimic βIII-tubulin rescues microtubule and cardiac defects in Duchenne muscular dystrophy mice.
Article Snippet: After 1 h of attachment onto laminin-coated coverslips (CellTreat 229,172) in 37 ◦C incubator, cardiomyocytes were chilled for 1.5 h in icy water to depolymerize microtubules, then rewarmed at 37 ◦C for the indicated periods (minutes): 0 (directly fix without warm-up), 1, 3, 5, 8, 10, 15. .. Cells were fixed in fixation buffer (4% paraformaldehyde, 60 mM PIPES, 25 mM HEPES, 10 mM EGTA, 1 mM MgCl2, 0.5% Triton X-100, pH 6.9) for 10 min at 37 ◦C, blocked with 1% BSA in PBS for 20 min at room temperature, and stained for α-tubulin (Abcam Ab7291, 1:1000, mouse) for 1 h at room temperature, followed by anti-mouse Alexa 555 (Invitrogen A21424, 1:250) for 1 h at room temperature [92]. ..

Article Title: IL-24 producing regulatory T and B lymphocytes in endometriosis
Article Snippet: Anti-human CD19 FITC (Biolegend, clone: HIB19), CD24 APC-Cy7 (Biolegend, clone: ML5), CD27 Pacific Blue (Biolegend, clone: M-T271), and CD38 Alexa Fluor 700 (Biolegend, clone: HB-7) antibodies were added for Bregs phenotyping, whereas anti-human CD4 Pacific Blue (Biolegend, clone: OKT4), CD25 APC (Invitrogen, clone: CD25-3G10), and CD127 Alexa Fluor 700 (Biolegend, clone: A019D5) antibodies were used for Tregs phenotyping. .. Samples were then washed with staining buffer (0.5 mM EDTA, 0.002% sodium azide, 1% fetal bovine serum) and fixed with a fixation buffer (eBioscience, USA) for 14 h at 4°C in the dark. .. After two washes with permeabilization buffer (eBioscience, USA), samples were blocked with Human True Stain FcX (Biolegend, USA) for 10 min at 4°C in the dark.